产品详情介绍
TransScript® Uni All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal)包含反转录反应所需的全部成分(TransScript® Uni RT, RNase Inhibitor, Anchored Oligo(dT)20 Primer, Random Primer(N9), dNTPs, Buffer)，可在同一管反应中实现cDNA 合成和去除基因组DNA。反应时，只需加入5×All-in-One Reaction Mix for qPCR、TransScript® Uni All-in-One Enzyme Mix、模板RNA和水，在42℃-65℃条件下即可高效地合成第一链cDNA，同时去除RNA模板中残留的基因组DNA 。另配有20×No RT Control Mix，用于配制无反转录酶的对照，判断qPCR模板是否来自cDNA。该产品操作简便，降低操作过程中的污染机率。cDNA产物只适用于qPCR，不适用于常规PCR。
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