产品详情介绍
TransScript® II Green Two-Step qRT-PCR SuperMix是具有高效合成能力和高扩增效率的两步法荧光定量PCR试剂盒。在同一反应体系中,用5×TransScript® II All-in-One SuperMix for qPCR和gDNA Remover在42℃-55℃条件下,高效地将RNA 合成第一链cDNA,同时去除基因组DNA;另配有5×TransScript® II All-in-One No-RT Control SuperMix for qPCR,用于配制无反转录酶的对照,判断qPCR模板是否来自cDNA。qPCR使用PerfectStart® Tip Green qPCR SuperMix扩增。
第一链cDNA合成和gDNA去除
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