产品详情介绍
本产品包含TransStart® FastPfu DNA Polymerase、dNTPs和优化的反应缓冲液，浓度为2×，扩增效率强，扩增速度快，具有高保真性、高特异性。DNA扩增时，只需加入模板、引物和水，使SuperMix溶液的浓度为1×即可进行反应。扩增产物为平端，可直接克隆于pEASY®-Blunt系列载体中。2×TransStart® FastPfu PCRSuperMix (+dye)扩增产物可直接点样电泳，如用于克隆，需纯化去掉染料。其PCR产物不适用于聚丙烯酰胺凝胶电泳。
• 减少PCR扩增操作时间。
• 避免因多步操作带来的污染。
• 保真性是EasyTaq® DNA Polymerase的54倍。
• 基因组DNA片段的扩增(≤15 kb)。
• Plasmid DNA片段扩增(≤20 kb)。
1 Song R, Guo P, Ren X, et al. A novel polypeptide CAPG-171aa encoded by circCAPG plays a critical role in triple-negative breast cancer[J]. Molecular Cancer, 2023.(IF 37.30)
2 Jin S, Lin Q, Gao Q, et al. Optimized prime editing in monocot plants using PlantPegDesigner and engineered plant prime editors (ePPEs)[J]. Nature Protocols, 2022.(IF 17.02)
3 Chen K, Hu Z, Song W, et al. Diversity of O-glycosyltransferases contributes to the biosynthesis of flavonoid and triterpenoid glycosides in Glycyrrhiza uralensis[J]. ACS Synthetic Biology, 2019.(IF 5.57)
4 Wang Y, Wang Z, Chen Y, et al. A highly efficient CRISPR-Cas9-based genome engineering platform in Acinetobacter baumannii to understand the H2O2-sensing mechanism of OxyR[J]. Cell Chemical Biology, 2019.(IF 6.76)
