产品详情介绍
2×EasyTaq® PCR SuperMix (-dye)包含EasyTaq® DNA Polymerase、dNTPs和优化的反应缓冲液，浓度为2×。DNA扩增时，只需加入模板、引物和水，使SuperMix溶液的浓度为1×即可进行反应。扩增产物3' 端带“A”碱基，2×EasyTaq® PCR SuperMix (-dye) 扩增产物可直接克隆于pEASY®-T系列载体中；2×EasyTaq® PCR SuperMix (+dye) 扩增产物可直接点样电泳，如用于克隆，需纯化去掉染料。其PCR产物不适用于聚丙烯酰胺凝胶电泳。
• 减少PCR扩增操作时间。
• 避免因多步操作带来的污染。
• 基因组DNA片段的扩增 (≤4 kb)。
1 Liu W, Yao Q, Su X, et al. Molecular insights into Spindlin1-HBx interplay and its impact on HBV transcription from cccDNA minichromosome[J]. Nature Communications, 2023.(IF 16.60)
2 Li C, Chen X, Wen R, et al. Immunocapture magnetic beads enhanced the LAMP-CRISPR/Cas12a method for the sensitive, specific, and visual detection of Campylobacter jejuni[J]. Biosensors, 2022.(IF 5.50)
3 Wang X, Liu C, Zhang S, et al. N6-methyladenosine modification of MALAT1 promotes metastasis via reshaping nuclear speckles[J]. Developmental cell, 2021.(IF 10.09)
4 Li D, Liu Y, Yi P, et al. RNA editing restricts hyperactive ciliary kinases[J]. Science, 2021.(IF 63.71)
5 Han D, Liu J, Chen C, et al. Anti-tumour immunity controlled through mRNA m6A methylation and YTHDF1 in dendritic cells[J]. Nature, 2019.(IF 64.8)
